2. Apoptosis
  3. Bcl-2 Family Apoptosis
  4. Bax inhibitor peptide V5

Bax inhibitor peptide V5  (Synonyms: BIP-V5; BAX Inhibiting Peptide V5)

目录号: HY-P0081 纯度: 99.77%
COA 产品使用指南

摩尔计算器

Bax inhibitor peptide V5 (BIP-V5) 是一种 Bax 诱导凋亡的抑制剂，主要用于癌症研究。

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Custom Peptide Synthesis

Bax inhibitor peptide V5 Chemical Structure

Bax inhibitor peptide V5 Chemical Structure

CAS No. : 579492-81-2

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5 mg ￥950
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10 mg ￥1400
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25 mg ￥3000
In-stock
50 mg ￥5000
In-stock
100 mg   询价  
200 mg   询价  

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Bax inhibitor peptide V5 相关抗体

Top Publications Citing Use of Products

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2018 Sep 15;355:269-285.  [Abstract]

    Bax inhibition ameliorates mitochondria dysfunction through modulating translocation and expression of Bax. H9c2 cells are treated with 160 nM TP for 24 h after pretreatment with BIP V5 (100 μM) or vehicle for 1 h. The cytoplasmic levels of Bax, Bcl-2, Cyt C, cleaved-caspase-9, cleaved-caspase-3 and β-actin are measured by immunoblot assays in H9c2 cells.

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2018 Sep 15;355:269-285.  [Abstract]

    Bax inhibition ameliorates mitochondria dysfunction through modulating translocation and expression of Bax. H9c2 cells are treated with 160 nM TP for 24 h after pretreatment with BIP V5 (100 μM) or vehicle for 1 h. The mitochondrial levels of Bax, Bak, Bcl-2, Cyt C, and COX VI are measured by western blot analysis in H9c2 cells.

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Sci Rep. 2017 Jul 3;7(1):4546.  [Abstract]

    Pretreatment with BIP-V5 significantly rescues cells from apoptosis induced by HDAC6 inhibition, whereas no effect is detected with a negative control peptide.

    Bax inhibitor peptide V5 purchased from MCE. Usage Cited in: Université Laval. 2017-08.

    Apoptosis is measured using Annexin V staining (green) in control (n=3 cell lines) and PAH-PASMCs (n=3 cell lines) pre-treated or not with Bax inhibitory peptides (BIP-V5, 200μM) or control peptides before being exposed to indicated HDAC6 inhibitors for 48 hours. Representative immunofluorescence images of apoptotic Annexin-V-positive cells (green) stained for DAPI (blue) and corresponding quantification are shown.

    查看 Bcl-2 Family 亚型特异性产品:

    查看所有亚型
    Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Bax inhibitor peptide V5 (BIP-V5) is a Bax-mediated apoptosis inhibitor, used for cancer treatment.

    IC50 & Target[1]

    Bax

     

    体外研究
    (In Vitro)

    Bax inhibitor peptide V5 (BIP-V5; 0-50 μM) reduces cell death in STF-cMyc cells but not in SW620 or NCI-H23 cells. BIPV5 does not result in any significant effect on cell cycle arrest at the G2/M phase[1]. V5 treatment upregulates expression of anti-apoptotic proteins Bcl-2 and XIAP by more than 3- and 11-fold and downregulates expression of apoptosis-inducing proteins Bax, Bad, and nuclear factor-κB-p65 by 10, 30, and nearly 50%, respectively[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Bax inhibitor peptide V5 相关抗体:
    体内研究
    (In Vivo)

    Bax inhibitor peptide V5 (BIP-V5; 100 μM) significantly improves islet function following isolation and improves islet graft function following transplantation in mice model[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    586.79

    Formula

    C27H50N6O6S
    CAS 号
    性状

    固体

    颜色

    White to off-white

    Sequence Shortening

    VPMLK
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : ≥ 100 mg/mL (170.42 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : 100 mg/mL (170.42 mM; 超声助溶)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.7042 mL 8.5209 mL 17.0419 mL
    5 mM 0.3408 mL 1.7042 mL 3.4084 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.26 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.26 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 100 mg/mL (170.42 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.77%

    COA (287 KB) LCMS (88 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [1]

    Cells (2.5×104 cells/mL) are grown in 96-well plates in a final volume of 100 μL/well. After 24 h, cells are incubated with small molecules or vehicle (DMSO) for 48-96 h prior to harvest. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole (MTT) reagent (0.5 mg/mL), is added to each well during the final 2 h, and absorbance is measured. Cell growth is calculated as the ratio of absorbance obtained upon compound treatment to that obtained with vehicle (DMSO) treatment.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Bax inhibitor peptide V5 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.7042 mL 8.5209 mL 17.0419 mL 42.6047 mL
    5 mM 0.3408 mL 1.7042 mL 3.4084 mL 8.5209 mL
    10 mM 0.1704 mL 0.8521 mL 1.7042 mL 4.2605 mL
    15 mM 0.1136 mL 0.5681 mL 1.1361 mL 2.8403 mL
    20 mM 0.0852 mL 0.4260 mL 0.8521 mL 2.1302 mL
    25 mM 0.0682 mL 0.3408 mL 0.6817 mL 1.7042 mL
    30 mM 0.0568 mL 0.2840 mL 0.5681 mL 1.4202 mL
    40 mM 0.0426 mL 0.2130 mL 0.4260 mL 1.0651 mL
    50 mM 0.0341 mL 0.1704 mL 0.3408 mL 0.8521 mL
    60 mM 0.0284 mL 0.1420 mL 0.2840 mL 0.7101 mL
    80 mM 0.0213 mL 0.1065 mL 0.2130 mL 0.5326 mL
    100 mM 0.0170 mL 0.0852 mL 0.1704 mL 0.4260 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

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